Pcr master mix calculator
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Tools: PCR Master Mix Calculator: Input: Results: Total Nr. of PCR Reactions Volume per reaction: µl Total volume µl DNA µl PCR buffer µl Primer 1 µl Primer 2 µl dNTP mix µl Enzyme µl MgCl 2 µl µl µl : µl DNA (Total) Total volume in master mix: µl H 2 O µl PCR buffer µl Primer 1 µl Primer 2 µl dNTP mix µl ...
We will set up an "assembly line" to add Master Mix and mineral oil before putting the tubes into the PCR machine. Add 40 uL of Master Mix plus the 10 uL already added to the PCR tube = 50 uL final reaction volume. MASTER MIX Reactant per Reaction x (number of students)+ 1 extra = Taq Polymerase 5 U/ul 0.1 uL ____ uL PCR buffer 10x 5 uL ____ uL